Adeno-X:

The Adeno-X™ Expression Systems offer the most efficient and effective method of adenoviral expression. These ligation-based or Cre recombination-based systems come with optimized protocols that let you obtain recombinant adenovirus in a few weeks. Adenoviral-mediated gene transfer produces the high protein expression levels in human cells (dividing or non-dividing cell types). This includes primary as well as established cultures. Because infected cells contain high numbers of episomal recombinant adenovirus, protein is transiently expressed at very high levels. Clontech also offer Adeno-X Tet-On® and Adeno-X Tet-Off® Expression Systems for inducible adenoviral protein expression.

Clontech also offer some convenient adenovirus tools, such as the Adeno-X Virus Purification Kit, Adeno-X Rapid Titer Kit, and Adeno-X Marker Viruses, which are designed to simplify and refine your adenoviral procedures.

Adeno-X Expression System 2 is a fast, efficient Creator™-based system for the constructing recombinant adenoviral DNA. This kit uses Cre-loxP site-specific recombination to transfer a target gene from a Creator Donor Vector to an adenoviral Acceptor Vector. The resulting recombinant adenoviral DNA can then be used to constitutively express a gene of interest in a wide variety of mammalian cells.

Retro-X:

Retroviral gene delivery is a highly efficient way of transferring genes into target cell populations. Using a single vector, you can infect most dividing cells with nearly 100% efficiency. Because your gene of interest integrates into the host cell genome, retroviral delivery produces heritable expression with low clonal variability while maintaining relative control over copy number.

The Retro-X™ Tet-On® and Retro-X™ Tet-Off® Advanced Inducible Expression Systems combine retroviral-mediated gene transfer with the most advanced inducible gene expression system. Tet-On and Tet-Off Advanced generate very low basal expression coupled with maximum fold-induction. These systems are designed to be used with our versatile Retro-X Universal Packaging System.

Retroviral Packaging Cell Lines and the Retro-X Universal Packaging System produce high titers of recombinant retroviruses able to infect the broadest range of cell types.

Retro-X Q Vectors harbor an optimized self-inactivating promoter in the 5' LTR that drives viral titers higher than those of other retroviral vectors, but eliminates promoter interference in infected target cells. Bicistronic expression of your transgene coupled with a selectable or fluorescent marker allows greater expression reliability in transduced cells.

Living Colors® Fluorescent Retroviral Vectors provide highly efficient, retroviral delivery of fluorescent protein markers, produce high titers, and allow you to tag or coexpress your protein of interest with a fluorescent protein to monitor cellular localization of your protein.

Lenti-X:

Recombinant lentiviruses derived from HIV-1 are able to deliver genes into almost any mammalian cell type, including primary cultures, nondividing cells, stem cells, and neurons. Clontech has developed an advanced lentiviral expression system that provides the broad cellular tropism of VSV-G pseudotyped lentivirus, extremely high viral titers, and excellent transgene expression.

pLVX-Puro

Lenti-X vectors, e.g. pLVX-Puro, not only carry the LTRs and packaging sequence required for lentivirus production and replication, but it also contain elements that improve transgene expression, titer, and overall vector function:

WPRE – The WPRE element, believed to promote RNA processing events and nuclear export (1), imparts a dual benefit.  First, it acts within the context of the viral genomic RNA to enhance vector packaging efficiency and increase the titers of viral supernatants produced from 293T packaging cells. Second, it boosts expression of your cDNA transgene in target cells by facilitating the production of mature mRNA from transcripts initiated by the vector’s internal CMV promoter

cPPT – The cPPT element increases nuclear importation of the viral genome during target cell infection, resulting in improved vector integration and more efficient transduction

Puromycin resistance – The pLVX-Puro vector’s puromycin resistance gene allows for rapid selection of transduced cells

Lenti-X HT Packaging System

The Lenti-X Expression System includes Clontech’s Lenti-X High Titer Packaging System, which generates virus of very high titers. You can often transduce an entire dish of target cells with just 10 ?l of unconcentrated viral supernatant. For more details, visit the Lenti-X HT Packaging System product pages.