ViraPower™:

Description:

The ViraPower™ Lentiviral and Adenoviral Expression Systems can be used for highly-efficient delivery of RNAi or expression constructs in mammalian cell lines, including primary cells and in vivo animal model systems. The high-titer viral stocks produced with these systems transduce target cells with a controlled number of viral particles, resulting in reproducible data. This makes these systems extremely useful for analyzing the role of protein expression, even in hard-to-transfect or non-dividing cell lines.

Long-term Stable or Transient Gene Expression?

The ViraPower™ Lentiviral Expression Systems stably integrate your gene of interest into your target cell's genome. By selecting with either Blasticidin or Zeocin™ selection agent, you can achieve high levels of constitutive or regulated stable gene expression from the CMV or UbC promoter, or stable expression of short hairpin RNA sequences from the U6 promoter. Youll overcome cell type limitations typically observed in other transfection protocols or with other viral delivery systems.

Gateway® Technology is used with the ViraPower™ Adenoviral Expression Systems so you can rapidly clone your gene or short hairpin RNA sequence of interest into an adenoviral vector. You'll get efficient transient gene expression and avoid lengthy, inefficient cloning procedures by using a simple one-hour recombination reaction. Both viral systems create replication-incompetent viral particles that can deliver and express your gene or short hairpin RNA sequence in essentially any mammalian cell type.

BLOCK-iT™ RNAi Products:

Description

BLOCK-iT™ Technology for RNAi analysis provides a selection of tools for a wide range of RNAi applications, including drug target validation, gene function analysis, and elucidation of molecular mechanisms of disease. With BLOCK-iT™ RNAi products you can quickly analyze knockdown of gene expression in mammalian cells. BLOCK-iT™ products provide you choices for introducing short interfering RNA (siRNA) into mammalian cells, including: In vitro transcription and dicing of double-stranded RNA (dsRNA), Synthetic siRNA, Vectors carrying a U6 cassette for transient or stable gene knockdown in dividing and non-dividing mammalian cell types.

RNAi Mechanism

RNA interference (RNAi) is a technique for blocking the translation of mRNA of a specific gene (1,2). In most eukaryotic cells, RNAi occurs by the cleavage of long double-stranded RNA (dsRNA) into 21-23 nucleotide short-interfering RNA (siRNA). These siRNA become part of an intracellular RNA-induced silencing complex (RISC) and cellular messenger mRNA that is complementary to the siRNA is targeted for degradation (Figure 1). Ultimately, this degradation of mRNA results in successful knockdown of gene expression in the selected cell or organism.

Reference(s):

1. Zamore, P.D. (2001) Nat. Struct. Biol. 8: 746-50.
2. Hannon, G.J. (2002) Nature 418: 244-51.